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Protein-Carbohydrate ZIP Conjugation Kit

SKU# KIT-1121S

Reactivity Kit to react a protein with a carbohydrate
Requirements Amine groups on Protein
Amine groups on Carbohydrate

Select a Size

1 Reaction enables you to conjugate 7.5 nmol of protein with 7.5 nmol of the carbohydrate or more, assuming you have at least 1 functional group per molecule. Vials are split into multiple reactions so that you can optimize between reaction sets

Regular price
$50.00
Regular price
$150.00
Sale price
$50.00
10 Available In Stock

Detailed Description

Save time, minimize headaches, and increase batch-to-batch consistency with our ZIP amine-amine conjugation kit.  

Within the kit, you'll receive all the materials you need to desalt both your protein and your carbohydrate, conjugate them together, and then rehydrate in a storage buffer of your choice. 

Unlike other conjugation kits, we also provide standards for comparison, so that you are confident of your results.

Kit contents:

  • 2 Types of Linkers, one for the protein and one for the carbohydrate
  • 1 Quencher
  • Amine-free Conjugation buffer
  • Filter vials for desalting and buffer exchange
  • Positive Control Protein Standard
  • Positive Control Carbohydrate Standard

Sizes:

  • With the Sample kit you can react your protein with your carbohydrate and compare to positive control standards. This kit allows you to react ~125 ug of your protein with ~75 ug of your carbohydrate**
  • The Medium kit allows you to run 3 reactions and 3 standards. You can react up to 375 ug of your protein together with 225 ug of your carbohydrate using this kit** 
  • The Large kit allows you to run 10 reactions and 10 standards. You can react up to 1.25 mg of your protein together with 750 ug of your carbohydrate.**

**Assuming you have a 17 kDa protein and a 10 kDa Carbohydrate

General procedure for using the kit:

  • Hydrate protein and carbohydrate in the provided amine-free buffer
  • Add linkers, leave for 1 hour
  • Mix together molecules that have reacted with linkers, leave for 1 hr
  • Done!

Products are for research use only

Protein-Carbohydrate ZIP Conjugation KitProtein-Carbohydrate ZIP Conjugation KitProtein-Carbohydrate ZIP Conjugation KitProtein-Carbohydrate ZIP Conjugation Kit